Well, i meant that you can watch files activities with dtrace. There`s a script which lets you monitor openings of specified file, and as folder is a file too, it might work - but, i`ve noticed it just now - it recreates itself on the reboot right ? If so, it wont help i guess because dtrace can provide you with info only while it`s running so i think it wont work.

If you want to check, type "opensnoop -f [path to the folder]" and leave it running, you shall see a diagram showing any app which is opening the file you specified. But still, i dont think it will work so the thing suggested by Naquuada will do better

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